Were imbibed in water, 0.05 M EDTA, 0.05 M CaCl2, or 0.5 MNa2CO3, stained with Ruthenium Red, and examined for seed mucilage abnormalities. EDTA is believed to loosen mucilage by disrupting the homogalacturonan salt bridges through Ca2+ chelation (Western et al., 2001; Rautengarten et al., 2008; Saez-Aguayo et al., 2013; Voiniciuc et al., 2013). Na2CO3 treatment also loosens mucilage, possibly by